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Abbrevia: Int. J. P P. Res.


Language: English


ISSN: 2315-6361


DOI: 10.14412/IJPPR



 Int. J. P P. Res.

3(2): 079-092, June 2020

 View: PDF (301KB) 




International Journal of Pharmacy and Pharmacology Research, Vol. 3(2), pp 079-092 June 2020


(ISSN 2315-6361) 2020 Science Park Journals

Full Length Research Paper



In Vivo Assessment of the Safety of Methanol Leaf Extract of Jatropha Gossypifoliaasan Antidiabetic Plant Using Hematological and Histopathologic Studies in Male Normal and Diabetic wistar Rats.


Uchendu A. P.1, Omogbai E. K. I.1*, Igbe I1, Uchendu J. O.2


1Department of Pharmacology and Toxicology, University of Benin, Benin City, Nigeria.

2Department of Morbid Anatomy, School of Medicine, Delta State University, Abraka, Delta State, Nigeria..





There is increased utility of herbal plants in the treatment of various diseases globally. In most cases, however, there is no scientific proof of their safety in humans. This study, which was to investigate the safety of using the methanol leaf extract of Jatropha gossypifolia (a plant widely used in Southern Nigeria for the treatment of various ailments particularly diabetes mellitus) by measuring its hematological and histopathological changes in male normal and diabetic wistar rats, is sequel to our earlier report on the pharmacological action of the same extract. Methanol leaf extract of J.gossypifolia at doses 100 and 200 mg/kg was given to the Wister rats orally for 14 days. On the 14th day, the blood samples of the study animals were collected and their livers, kidneys, lungs and hearts were also harvested and processed using standard techniques. Results showed no significant adverse changes in hematological parameters but a restoration of the red blood cell count of diabetic rats to normality. There were also no significant histopathological changes in the tissue sections of lungs, kidneys, and heart of rats when compared to control animals. However, significant dose-related lymphocytic infiltration and necrosis were seen in the liver sections of the rats treated with the extract, compared to control. The study shows that the extract is hepatotoxic and this therefore calls for caution in its use as medication and the need for proper in vivo assessment of the toxicity of the herbal extract before approval is given for its use by Government regulatory bodies either as a remedy for specific ailments or as a food supplement.


Key words:Jatropha gossypifolia, methanol leaf extract, safety, diabetes mellitus

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