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Abbrevia: S. P. J. App Micro Res.

 

Language: English

 

ISSN: 2315-6287

 

DOI: 10.14412/SPJAMR

 

 S. P. J. App Micro Res.

4(1): 29-58, May 2019

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Science Park Journal of Applied Microbiology Research,

Vol. 4 (1), pp 029-058, May 2019

doi: 10.14412/JARNR2019.058

(ISSN 2315-6260) 2019 Science Park Journals

 

Full Length Research Paper

 

Detection the Distribution of Ureaplasma parvum in Women with Recurrent Miscarriage  by Polymerase Chain Reaction Assay. 

 


Ghofran K. Al-khafaji

 

Technical Institute / Samawa / Al-Furat Al-Awsat Technical University / Samawa / Iraq.

 
Abstract

One hundred seventy specimen included vaginal bleeding , vaginal swab and urine , were collected One hundred thirty specimen from women with recurrent miscarriage and fourtyspecimen from control. Through the research two types of media were used (IH broth medium) and (IH agar medium). positive isolates of Ureaplasma spp. in culture were investigated by PCR assay to identification of Ureaplasma parvum and subtyping to (SV1, SV3, SV6, SV14). The results showed the Ureaplasma parvum was identified in (29.6%) from patient and (11%) from control. Ureaplasma parvum isolates were further subtyped by using PCR assay, the results revealed the serovar3 was the most repeater isolate in rate (42.8%), while serovar1(28.5%), serovar6 (14.2%) and serovar14 (14.2%) in patient but in control only serovar1 was isolated in rate (11%). These results indicate that Ureaplasma parvum infection may be an important etiologic agent of recurrent miscarriage and serovar3 was the most repeater serovar detected in present study.

Keywords: Ureaplasma parvum , serovars, IH medium ,recurrent abortion , PCR, subtyping.


 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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