Detection the Distribution of
Ureaplasma parvum in Women with
Recurrent Miscarriage by Polymerase
Chain Reaction Assay.
Ghofran K. Al-khafaji
Technical Institute / Samawa / Al-Furat
Al-Awsat Technical University / Samawa /
hundred seventy specimen included vaginal
bleeding , vaginal swab and urine , were
collected One hundred thirty specimen from
women with recurrent miscarriage and
fourtyspecimen from control. Through the
research two types of media were used (IH
broth medium) and (IH agar medium). positive
isolates of Ureaplasma spp. in culture were
investigated by PCR assay to identification
of Ureaplasma parvum and subtyping to (SV1,
SV3, SV6, SV14). The results showed the
Ureaplasma parvum was identified in (29.6%)
from patient and (11%) from control.
Ureaplasma parvum isolates were further
subtyped by using PCR assay, the results
revealed the serovar3 was the most repeater
isolate in rate (42.8%), while
serovar1(28.5%), serovar6 (14.2%) and
serovar14 (14.2%) in patient but in control
only serovar1 was isolated in rate (11%).
These results indicate that Ureaplasma
parvum infection may be an important
etiologic agent of recurrent miscarriage and
serovar3 was the most repeater serovar
detected in present study.
Ureaplasma parvum , serovars, IH medium
,recurrent abortion , PCR, subtyping.
This is an open access
article published under the terms of the Creative
Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium,
provided the original work is properly cited.