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Abbrevia: S. P. J. App Micro Res.


Language: English


ISSN: 2315-6287


DOI: 10.14412/SPJAMR


 S. P. J. App Micro Res.

4(1): 17-28, May 2019

 View: PDF (190KB) 

 Search Authors Articles in:


Makut,M. D


Ombugadu, A

Abikoye, E.T

Mohammed S.A


Science Park Journal of Applied Microbiology Research,

Vol. 4 (1), pp 017-028, May 2019

doi: 10.14412/JARNR2019.028

(ISSN 2315-6260) 2019 Science Park Journals


Full Length Research Paper


Isolation, identification andScreening of Pseudomonas fluorescens from soil environment of Keffi, Nasarawa state - Nigeria for the production of Bio-fertilizer.


Makut, M. D.1,Ishaya,I.O.1*, Ombugadu, A.2, Onawola,O.O.3, Abikoye, E.T.3, Mohammed S.A3

1Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa State University Keffi, P.M.B 1022, Nasarawa State.

2Department of Zoology, Faculty of Science, Federal University Lafia, P.M.B. 146, Lafia, Nasarawa State.

3Federal Institutes of Industrial Research Oshodi, P.M.B. 21023, Lagos.


Bio-fertilizer has been identified as an alternative to chemical fertilizer to increase soil fertility and crop production with no residual harmful effect to the environment.This study on isolation, identification andscreening of Phosphate solubilisationabilityofPseudomonas fluorescens from soil environmentwas carried out from May to September 2018. Soil samples were collected from eight different locations and Pseudomonas strainswere isolated and identified using standard microbiological methods.The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Pseudomonas fluorescensreference type straindeposited in RDP GenBank. Pseudomonas strains isolated from five different sites showed clear halo zone ranging from 8-18mm. Solubilized Phosphate content produced by Pseudomonas ranged from 207.3 mg/l -273.75 mg/l.

Keywords: Bio-fertilizer, Pseudomonas fluorescens,Phosphate solubilisation


This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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