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Abbrevia: S. P. J. App Micro Res.

 

Language: English

 

ISSN: 2315-6287

 

DOI: 10.14412/SPJAMR

 

 S. P. J. App Micro Res.

4(3):101-113, October 2019

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Science Park Journal of Applied Microbiology Research,

Vol. 4 (3), pp 101-113, October 2019

doi: 10.14412/SPJAMR2019.113

(ISSN 2315-6260) 2019 Science Park Journals

 

Full Length Research Paper

 

Effect of Optimization Conditions on Submerged Fermentation of Corn bran for the production of xylanase enzyme using Mutant strain of Aspergillus niger.

 

Afangide, C.S1*, Orukotan A. A1, Ado, S.A 2 ,  Egbe, L3

 

1Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria.\

2Department of Microbiology, Faculty of Science, Amhadu Bello University, Zaria, Nigeria.

 3Department of Biological Sciences, Faculty of Science, Nigeria Defence Academy, Nigeria

 

 
Abstract

UV- rays were used as mutagens on wild type of Aspergillus niger for enhanced production of Enzyme – Xylanase. The xylanase producing species was isolated from soil and exposed to UV rays at various timings of 20mins, 40mins, 60mins, 80mins, 100mins and 120mins.  The mutants showed 19.96, 21.22, 22.66, 27.88, and 37.77 %, increase in activities of cellular xylanase respectively in comparison with the parental strain. Mutant AN120 exposed to UV rays for 120 minutes showed a decrease in enzyme activity (0.444 0.04) in comparison with the parent strain (0.556 0.06 ). Mutant AN100 exposed to UV ray for 100minutes was therefore selected as the best producer of xylanase after 7 days of fermentation using Corn bran as a substrate. Optimization of xylanase production was carried out and the result obtained indicated that mutant AN100 showed best xylanase activity when cultivated at pH 5, 30oC and without addition of any other Carbon or Nitrogen source. Aspergillus niger is a good producer of xylanase under the conditions determined in this assay

 

Keywords: UV- rays, , Enzyme – Xylanase , Aspergillus niger,

 

   


 

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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